The draw for the fourth round of the Tetley's Challenge Cup will take place at 3.45pm on Tuesday March 18 from MediaCityUK in Manchester.
The fourth round sees the entry of the 14 Super League clubs, who join the 18 winners of this weekend's third-round ties in a competition that will climax with the final at Wembley Stadium on Saturday August 23.
Wigan Warriors are the current holders of Rugby League's oldest and most prestigious knockout competition, which was first contested in 1897.
The Warriors, who have the most final appearances and victories to their name, blanked Hull FC 16-0 in last year's final at Wembley while Warrington beat Leeds in the 2012 showdown.
The home teams will be drawn by the Mayor of Wigan, Councillor Billy Rotherham; the away teams will be drawn by David Barker, representing sponsors Tetley's.
The fourth round of the Tetley's Challenge Cup will be played over the weekend of April 5-6.